Optimization of Photoperiod Treatment for Enhanced Biomass Production and Primary Metabolite Content in Euglena sp. Strain Isolated from Yogyakarta, Indonesia

Abstract

The cultivation of microalgae as an alternative energy resource requires the optimization of the cultivation process. Euglena is a biofuel microalgae with proteins, essential amino acids, vitamins C and E, lipids, beta carotene, and beta-1,3-glucan (paramylon). Light intensity and photoperiod are important factors in determining microalgae biomass production because of their relationship to maximum photosynthesis efficiency. As a result, optimizing growth conditions in microalgae cultivation is critical for maximizing productivity. This study looked at how different photoperiod treatments (12:12, 14:10, 16:8, 18:6, and 24:0 h) affected the growth, biomass, lipid, carbohydrates, and protein content of Euglena sp. cultures in modified CM medium. The 18:6 photoperiod treatment produced the highest specific growth rate, biomass, and lipid carbohydrate, and protein content, according to the study's findings The biomass concentration was 0.4190.184 g/L, and the specific growth rate was 5.960.66 (OD680/OD680/hx10-3). The lipid concentration was 0.2030.078 g/L, the carbohydrate concentration was 378.061252.135 mg/mL, and the protein concentration was 371.142107.126 g/L.