Exploring the Combined Efficacy of 3-Hydrazinoquinoxaline-2-thiol and Flucloxacillin Against Methicillin-Resistant Staphylococcus aureus Efficacy of 3-Hydrazinoquinoxaline-2-thiol and Flucloxacillin Against Methicillin-Resistant Staphylococcus aureus

Abstract

The escalating resistance observed in different antimicrobial agent, especially those considered as last-line options, highlights the urgent required for innovative ways and novel agents to combat MRSA infections. Utilizing antibiotic combinations can enhance efficacy, broaden the spectrum against bacteria, and mitigate the risk of resistance development, offering a promising strategy to address the rising challenge of antimicrobial resistance. This paper aims to explore the probability of synergistic effects between 3-hydrazinoquinoxaline-2-thiol (3HX) and flucloxacillin (FLX) versus a different range of MRSA clinical isolates, which may provide valuable insights into their the combination of the antimicrobial activity. Broth microdilution was conducted on 22 clinical MRSA isolates to assess the Minimum Inhibitory Concentrations (MICs) of both 3HX and FLX. Following by a checkerboard assay was subjected to assess the interaction activity between the two agents, focusing on the Fractional Inhibitory Concentration Index (FICI). The MICs of FLX and 3HX were evaluated for 22 clinical MRSA strains, with FLX displaying MICs ranging from 128 to 512 µg/ml, while 3HX MICs varying from 16 to 64 µg/ml. Surprisingly, the combinationof3HX and FLX demonstrated a synergistic effect, leading to a considerable reduction of MIC sbyup to 64-fold. The potential of combining 3HX with FLX as an effective way versus MRSA appears promising. But, further rigors testing and experimentation are imperative to establish its practical utility.